Assembly Settings¶
Assembler (Required)¶
Choose an assembler tool from the select list.
Canu¶
Genome Size (Required)¶
Set the estimated genome size.
Note
- e.g. 5m or 2.6g, which is used for solid k-mers selection.
- The estimate could be rough (e.g. within 0.5x-2x range) and does not affect the other assembly stages.
Flye¶
Genome Size (Required)¶
Set the estimated genome size.
Note
- e.g. 5m or 2.6g, which is used for solid k-mers selection.
- The estimate could be rough (e.g. within 0.5x-2x range) and does not affect the other assembly stages.
Unicycler¶
Short 1 (Optional)¶
Set the path to the fastq file of first short reads in each pair.
Short 2 (Optional)¶
Set the path to the fastq file of second short reads in each pair
Note
- These two options are required in hybrid assembly mode, or leave it blank for long-read-only assembly mode.
- These two fastq files should be obtained from Illumina sequencing.
[1] | Canu Tutorial https://canu.readthedocs.io/en/latest/tutorial.html |