Assembly Settings

Assembler (Required)

Choose an assembler tool from the select list.

Canu

Genome Size (Required)

Set the estimated genome size.

Note

  • e.g. 5m or 2.6g, which is used for solid k-mers selection.
  • The estimate could be rough (e.g. within 0.5x-2x range) and does not affect the other assembly stages.

assembly-prefix [1] (Default)

Note

  • Set value: asm.
  • Set the file name prefix with “asm” of intermediate and output files.

Flye

Genome Size (Required)

Set the estimated genome size.

Note

  • e.g. 5m or 2.6g, which is used for solid k-mers selection.
  • The estimate could be rough (e.g. within 0.5x-2x range) and does not affect the other assembly stages.

Unicycler

Short 1 (Optional)

Set the path to the fastq file of first short reads in each pair.

Short 2 (Optional)

Set the path to the fastq file of second short reads in each pair

Note

  • These two options are required in hybrid assembly mode, or leave it blank for long-read-only assembly mode.
  • These two fastq files should be obtained from Illumina sequencing.
[1]Canu Tutorial https://canu.readthedocs.io/en/latest/tutorial.html